Agrobacterium-mediated transformation of rough lemon (Citrus jambhiri Lush) with yeast HAL2 gene
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چکیده
منابع مشابه
Evaluation of rough lemon (Citrus jambhiri Lush.) as rootstock for salinity tolerance at seedling stage under in vitro conditions
In vitro approach was adopted to study the effect of salinity on survival and growth of rough lemon (Citrus jambhiri Lush.) seeds. North-West (Punjab) part of India has been facing a major problem of soil salinity for citrus orchards. Therefore, it is logical to study the salinity tolerance of common citrus rootstock, rough lemon (Citrus jambhiri Lush.), grown in the region. The seeds were trea...
متن کاملCharacterization of a sabinene synthase gene from rough lemon (Citrus jambhiri).
We previously isolated two putative monoterpene synthase genes, RlemTPS1 and RlemTPS2, from rough lemon (Citrus jambhiri) and showed that gene expression of RlemTPS2 was induced by microbial attack. The protein product of RlemTPS2 was obtained using a prokaryotic expression system, and GC and GC-MS of monoterpene synthesis by RlemTPS2 determined that RlemTPS2 encodes a sabinene synthase. Sabine...
متن کاملAgrobacterium tumefaciens-mediated transformation of yeast.
Agrobacterium tumefaciens transfers a piece of its Ti plasmid DNA (transferred DNA or T-DNA) into plant cells during crown gall tumorigenesis. A. tumefaciens can transfer its T-DNA to a wide variety of hosts, including both dicotyledonous and monocotyledonous plants. We show that the host range of A. tumefaciens can be extended to include Saccharomyces cerevisiae. Additionally, we demonstrate t...
متن کاملAgrobacterium - Mediated Transformation of Citrus Cell Suspension Cultures
An efficient protocol for the genetic transformation of cell suspension cultures of several citrus cultivars using Agrobacterium is described. Cell suspension cultures of Citrus sinensis ‘Hamlin’, ‘Valencia’ and ‘OLL8’ (an early maturing ‘Valencia’ like somaclone), Citrus unshiu ‘Okitsu wase’ and Citrus reticulata ‘Ponkan’ and ‘W. Murcott’ were transformed using A. tumefaciens EHA105. It was de...
متن کاملA Preliminary Experiment on Agrobacterium tumefaciens-Mediated Transformation of the P5CS1 GENE in Tall Fescue
Abiotic stress conditions can be imposed on plants through either poor water quality or adverse climate condition. These types of stress affect plant growth and development throughout their life cycle. Plants that are tolerant to drought are therefore valuable, and turf grasses have the potential to grow tolerant. Turf grasses provide ground cover and offer multiple benefits, from their aesthet...
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ژورنال
عنوان ژورنال: BMC Research Notes
سال: 2012
ISSN: 1756-0500
DOI: 10.1186/1756-0500-5-285